Isolation of Lactic Acid Bacteria (LAB) from Digestive Tract of Asian Swamp Eel (Monopterus albus) and Bacterial Antagonist Test to Inhibit Pathogenic Bacteria Aeromonas hydrophila and Pseudomonas sp

Rudi Alfinda, Iesje Lukistyowati, Henni Syawal, Iskandar Putra

Abstract


Lactic Acid Bacteria (LAB) are probiotic-forming bacteria that have characteristics, like be able to ferment sugar or carbohydrates and produce lactic acid as the final product of fermentation. The purpose of this study was to obtain isolates of lactic acid bacteria from the digestive tract of Asian Swamp Eel (Monopterus albus) and to determine the inhibition produced by these lactic acid bacteria in inhibiting pathogenic bacteria such as Aeromonas hydrophila and Pseudomonas sp. The method was used in this study is experimental method, by taking the digestive tract of Asian Swamp Eel and then identifying the lactic acid bacteria and characterized by morphological and biochemical characteristics and tested of the antagonist against the pathogenic bacteria Aeromonas hydrophila and Pseudomonas sp. using the Kirby Bauer disc method (disc method). From the research results obtained 5 types of candidate lactic acid bacteria with each code BL121, BL263, BL142, BL242 and BL342. The five isolates of lactic acid bacteria were able to inhibit Aeromonas hydrophila and Pseudomonas sp. with a Inhibition range of 8-16 mm. Based on the results of the study, there were three isolates that had inhibitory power against bacteria A. hydrophila (12-16 mm) and Pseudomonas sp (11-13 mm) with codes BL121, BL263 and BL142 and were classified as strong. The identification results obtained are bacteria from the genus Bacillus sp

Keywords


Asian Swamp Eel; Pathogenic Bacteria; Antagonist Test; Lactic Acid Bacteria

Full Text:

PDF

References


Abun. (2008). Hubungan Mikroflora dengan Metabolisme dalam Saluran Pencernaan Unggas dan Monogastrik. Makalah Ilmiah. Fakultas Peternakan. Universitas Padjadjaran.

Akpinar, A., Yerlikaya, O., Kilic, S. (2011). Antimicrobial Activity and Antibiotic Resistance of Lactobacillus delbrueckii ssp, Bulgaricus and Streptococcus thermophilus Strain Isolated from Turkish Homemade Yogurts. African Journal of Microbiology Research. 5(6): 675-682.

Aslim, B., Z.N. Yuksekdag, E. Sarikaya, Y. Beyatli. (2005). Determination of The Bacteriocin-Like Substances Produced by Some Lactic Acid Bacteria Isolated from Turkish Dairy Products. LWT. 38 : 691-694.

Balcazar, J.L., de Blas, I., Ruiz., Zanzuela, I., Vendrell, D., Girones, O., Muzquiz, J.L. (2008). Enhancement of the Immune Response and Protection Induced by Probiotic Lactic Acid Bacteria Against Furunculosis in Rainbow Trout (Oncorhynchus mykiss). Journal Article, Research Support, Non-4.S. Gov’t.

Campos, A., O. Rodriguez, P. Calo-Mata, M. Prado and J. Barros-Velazquez. (2006). Preliminary characterization of bacteriocins from Lactococcus lactis, Enterococcus faecium, and Enterococcus mundtii strains isolated from turbot (Psetta maxima). Food Research International, 39: 356-364

Cappuccino, J.G., Sherman, N. (2001), “Microbiology: A Laboratory Manual”. New York: Addison-Wesley Publishing Company.

Desniar., Rusmana, I., Suwanto, A., Mubarik, N.R. (2012). Senyawa Antimikroba yang Dihasilkan oleh Bakteri Asam Laktat Asal Bekasam. Jurnal Akuatika. 3(2): 135-145.

Dewi, I.M. (2008). Isolasi Bakteri dan Uji Aktifitas Kitinase Termofilik Kasar dari Sumber Air Panas Tinggi Raja, Simalungun, Sumatera Utara. Tersedia dari Tesis. Sekolah Pascasarjana Universitas Sumatera Utara, Medan

Feliatra., Effendi, I., Suryadi, E. (2004). Isolasi dan Identifikasi Bakteri Probiotik dari Ikan Kerapu Macan (Ephinephelus fuscoguttatus) dalam Upaya Efisiensi Pakan Ikan. Jurnal Natur Indonesia. 6(2): 75-80.

Guerra, N.P., Bernardez, P.F., Mendez, J., Cachaldora, P., Castro, L.P. (2006). Production of Four Potentially Probiotic Lactic Acid Bacteria and Their Evaluation as Feed Additives for Weaned Piglets. Animal Feed Science and Technology. 134: 89-107.

Guessa, B., & Kihal, M. (2004). Characterization of Lactic Acid Bacteria Isolated from Algerian Arid Zone Raw Goats’ Milk. African Journal of Biotechnology. 3(6):339-342.

Harmita, & Radji, M. (2004). Analisis Hayati. Jakarta: Departemen Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia. pp. 62-3.

Hasanah, U. (2014). Bakteri Asam Laktat dari Daging Ikan Peda sebagai Agen Probiotik dan Enzim Kolesterol Reduktase. Jurnal Keluarga Sehat Sejahtera. 12(23):1-8

Hatmanti, A. (2000). Penganalan Bacillus spp. Oseana, Vol. XXV(1): 31-41

Holt, J.G., N.R. Kreig, P.H.A. Sneath, J.T. Staley, & S.T. Williams. (2000). Bergey’s Manual of Determinative Bacteriology. Ninth Edition. Lippincott Williams and Wilkins, Philadelphia.

Holzapfel, W.H.P., Haberer, R., Geisen, J., Bjorkroth., Schillinger. (2001). Taxonomy and Important Features of Probiotic Microorganism in Food and Nutrition. The American Journal of Clinical Nutrition.

Imadibrata, M. (2010). Probiotik-Peranannya dalam Dunia Medis. Universitas Indonesia. Jakarta.

Indriani, N. (2007). Aktivitas Antibakteri Daun Senggugu (Cleodendron serratum L). Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Pertanian Bogor. Bogor.

Iribarren, D., P. Dagá. M. T. Moreira., G. Feijoo. (2012). Potential environmental effects of probiotics used in aquaculture. Aquacult Int, 20, 779-789

Junariati, M.F. (2009). Panen Belut 3 Bulan di Media Air Bening Tanpa Lumpur. Penebar. Jakarta.

Kordi, M.G.H. (2014). Budidaya Belut di Media Air Secara Organik. Penerbit Lily Publisher. Yogyakarta.

Madigan, M.T. & Martinko, J.M. (2006). Biology of Microorganism. 9th Edition. Prentice Hall International, Inc. New Jersey.

Mansur, A., Tangko, A.M. (2008). Probiotik : Pemanfaatannya untuk Pakan Ikan Berkualitas Rendah. Balai Riset Perikanan Budidaya Air Payau. Jurnal Akuakultur. 3(2): 145-149.

Pitaloka, L., Lukistyowati, I., Nursyirwani. (2015). Isolasi Bakteri Kandidat Probiotik dari Usus Ikan Gurami (Osphronemus gouramy Lac.) untuk Pengendalian Aeromonas hydrophila. Jurnal Online Mahasiswa (JOM).

Sarbaini. (2015). Isolasi Bakteri Kandidat Probiotik dari Usus Ikan Nila (Oreochromis niloticus) untuk Pengendalian Streptococcus agalactiae. Tersedia dari Pekanbaru: Universitas Riau.

Subagiyo., Margino, S., Triyanto., Setyati, W.A. (2015). Pengaruh pH, Suhu dan Salinitas Terhadap Pertumbuhan dan Produksi Asam Organik Bakteri Asam Laktat yang Diisolasi dari Intestinum Udang

Penaeid. Ilmu Kelautan, 20(4): 187-194.

Sharah, A., Karnila, R., Desmelati. (2015). Pembuatan Kurva Pertumbuhan Bakteri Asam Laktat yang Diisolasi dari Ikan Peda Kembung (Rastrelliger sp.). JOM. 1-19.

Susana, M., Feliatra., Lukistyowati, I. (2017). Isolasi dan Karakterisasi Bakteri Heterotrofik pada Perairan Laut Kawasan Pemukiman dan Perairan Bersalinitas Rendah di Kelurahan Purnama Dumai Provinsi Riau. JOM 1-16

Sulistijowati, S.R., Mile, L. (2016), Identification of Lactic Acid Bacteria Isolates from Intestine of Milkfish (Chanos chanos) Potential Activity Against Pathogen Bacteria Used PCR 18s Rrna Methode. International Journal Bio-Science and Bio-Technology, 8(3), 127-134.

Situmeang, S.M.F., Musthari, & Riadi, S. (2017). Isolasi dan Uji Aktivitas Antimikroba Bakteri Asam Laktat (BAL) dari Yoghurt dalam Menghambat Pertumbuhan Bakteri Escherichia coli dan Salmonella typhi. Jurnal Biosains. 3(3):144-152.

Tome, Y., P. Teixeira, & P.A. Gibbs. (2006). Anti-Listerial Inhibitory Lactic Acid Bacteria Isolated from Commercial Cold Smoked Salmon. Food Microbiol. 23: 399-405.

Vazquez, J.A., M.P. Gonzales, & M.A. Murado. (2005). Effects of Lactic Acid Bacteria Cultures on Pathogenic Microbiota from Fish. Aquaculture. 245: 149-161.

Verschuere, L., Rombaut, G., Sorgeloos, P., Verstraete, W. (2000). A Probiotic Bacteria as Biological Control Agents in Aquaculture. Microbiology And Molecular Biology Review 64: 2527-2533.

Willey, J.M., Sherwood, L.M., & Woolverton, C.J. (2009). Prescott’s Principles of Microbiology. Boston: McGraw-Hill Higher Education

Yulvizar, C. (2013). Isolasi dan Identifikasi Bakteri Probiotik Pada Rastrelliger sp. Biospecies. 6(2):1-7.

Yusra, Azima, F., Novelina & Periadnadi. (2010). Isolasi dan Identifikasi Mikroflora Indigenous dalam Budu. Jurnal Agritech. 34(3): 316-321.




DOI: http://dx.doi.org/10.31258/jpk.27.3.412-420

Copyright (c) 2022 Rudi Alfinda, Iesje Lukistyowati, Henni Syawal, Iskandar Putra

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.


Gedung Marine Center Lt 2. Fakultas Perikanan dan Kelautan Universitas Riau

 Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.