Growth Analysis of Single Cell Protein (PST) Bacteria Bacillus cereus using Different Media

Mutiara Naimi Nasution, Feliatra Feliatra, Irwan Effendi

Abstract


The Media is a material consisting of a mixture of nutrients used by microorganisms to grow and breed. Molasses and liquid waste know one of the alternative media which has the least inexpensive carbon source and is easily obtained from the waste food industry that is rich in nutrients and minerals, so it has the potential for bacterial growth of Bacillus cereus. The consetration of molasses used for growth medium is 1%; 1.5%; and 2% while liquid waste knows 8%, 10%, and 12%. Cell growth is measured by the method of Spectrophotometry, total plate count (TPC) and biomass while the incubation period of cell growth is measured every 0, 6, 12, 18, and 24 hours. The results showed from the molasses + skim milk and the tofu liquid waste + skim milk, the best medium of bacterial cell growth of B. cereus is a liquid waste know isolates K 12%. While the best growth concentrations molasses Medium bacterial B.cereus was N2% and K2%.


Keywords


Bacillus cereus; Molasses; Single Cell Protein; Tofu Liquid Waste

Full Text:

PDF

References


Baek, J.G., S.M. Shim., D.Y. Kwon., H.K. Choi., C.H. Lee., and Y.S. Kim. 2010. Metabolite Profiling of Cheonggukjang, A Fermented Soybean Paste, Inoculated With Various Bacillus Strains During Fermentation. Biosci. Biotechnol. Biochem. 74:1860–1868.

Damanik, Y., N. Hidayat., dan S. Anggarini. 2014. Pengaruh Penambahan Molase dan Lama Waktu Fermentasi Pada Kualitas Teh Kompos Sebagai Biobakterisida Terhadap Pengendalian Bakteria Ralstonia Solancearum. Skripsi. Universitas Brawijaya.

Feliatra., Nursyirwani., A. Tanjung., D.S. Adithiya., M. Susanna., dan I. Lukystyowati. 2018. Effectiveness of Heterotrophic Bacteria Isolated from Dumai Marine Waters of Riau, Used as Antibacterial against Pathogens in Fish Culture. Earth and Environmental Science. 116:1-12.

Hamdiyati, Y. 2014. Pertumbuhan dan Pengendalian Mikroorganisme II. Jakarta.

Hidayati, A.D. 2017. Pengaruh Hasil Reformulasi dengan Penambahan Kh2po4 dan NH4Cl pada MHB (Mycorrhiza Helper Bacteria) dan Glomus Spp. Terhadap Populasi Pratylenchus Coffeae dan Pertumbuhan Bibit Kopi Serta Pemanfaatannya Sebagai Buku Nonteks. Skripsi. Universitas Jember.

Manfaati, R. 2010. Kinetika dan Variable Optimum Fermentasi Asam Laktat dengan Media Campuran Tepung Tapioka dan Limbah Cair Tahu oleh Rhizopus oryzae. Tesis. Magister Teknik Kimia. UNDIP. Semarang.

Madigan, M. T., J.M. Martinko., D. Stahl., and Clark. 2012. Brock Biology of Microorganisms (13th Edition). New York: Pearson.

Pengkumsri, N., B.S. Sivamaruthi., S. Sirilun,. S. Peerajan, P. Kesika., K. Chaiyasut., and C. Chaiyasut. 2017. Extraction of β-glucan from Saccharomyces cerevisiae : Comparison of Different Extraction Methods and in Vivo Assessment of Immunomodulatory Effect in Mice. Food Science and Technology. 7(1):124–130.

Purwaningtyas, Y.R. 2019. Produksi Protein Sel Tunggal Gluconacetobacter xylinus Dengan Medium Limbah Cair Tempe Menggunakan Metode Air - Lift Bioreactor. Skripsi. Universitas Sanata Dharma. Yogyakarta.

Quinn, P. J., B.K. Markey., F.C. Leonard., E.S. Fitzpatrick., S. Fanning., and P. Hartigan. 2011. Veterinary Microbiology and Microbial Disease. Oxford: Willey-Blackwell Ltd.

Rochani, A., S. Yuniningsih., dan Z. Ma’sum. 2016. Pengaruh Konsentrasi Gula Larutan Molases Terhadap Kadar Etanol pada Proses Fermentasi. Jurnal Reka Buana. 1(1):43-48.

Somaye, F., M.N. Marizieh., and N. Lale. 2008. Single Cell Protein (SCP) production from UF cheese when by Kluyveromyces marxianus. Iran: 18th National Congresson Food Technology.

Suminto. 2008. Pertumbuhan Bakteri Probiotik Alkaligenus sp. dan Flavobacterium sp. yang Diisolasi dari Usus Udang pada Media Kultur Molase dan Kaolin. Jurnal Saintek Perikanan. 4(1): 21 – 27.

Wahyuningsih, N dan E. Zulaika. 2018. Perbandingan Pertumbuhan Bakteri Selulolitik pada Media Nutrient Broth dan Carboxy Methyl Cellulose. Jurnal Sains dan Seni Its. 7(2):2337-3520.

Walker, G., and G. Stewart. 2016. Saccharomyces cerevisiae in the Production of Fermented Beverages. Beverages. 2:1–12.

Wibowo, M.S. 2016. Faktor yang Mempengaruhi Pertumbuhan Mikroorganisme. School of Pharmacy ITB.

Wijanarka., E. Kusdiyanti., and S. Parman. 2016. Screening Cellulolytic Bacteria from The Digestive Tract Snail (Achatina fulica) and Test the Ability of Cellulase Activity. Biosaintifika. 8(3):386–39.




DOI: http://dx.doi.org/10.31258/jpk.26.1.47-53

JURNAL PERIKANAN DAN KELAUTAN Gedung Marine Center Lt 2. Fakultas Perikanan dan Kelautan Universitas Riau

 

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.