Sensitivity of Terminalia catappa L. Toward Aeromonas hydrophila
(1) Universitas Riau
(2) Universitas Riau
(3) Universitas Riau
(*) Corresponding Author
Abstract
Terminalia catappa is commonly present in tropical area and its can be used for increasing pH in fish culture and it also be used as disinfectant. A research aims to understand the dosages of T. catappa leaf extract to hamper the growth of Aeromonas hydrophila has been conducted from February to July 2019. A diffusion agar KIRBY BAUER method was applied to understand the sensitivity of T. catappa toward the A. hydrophila. Treatments applied were P0 (Control, Oxytetracyclin (30 µg.Disk), P1 (100%), P2 (90%), P3 (80%), P4 (70%), P5 (60%), P6 (50%), P7 (40%), P8 (30%), P9 (20%), P10 (10%) P11 (9%), P12 (8%), P13 (7%), P14 (6%), P15 (5%), P16 (4%), P17 (3%), P18(2%), P19 (1%). Pure leaf extract was obtained from fresh leaf. The disk blank was wet with 50 L of leaf extract. A. hydrophila was cultured in TSA, TSB and GSP media, and it was grew up for 24 hours. Results shown that the pure leaf extract produce 13.9 mm diameter clear zone, while 2% of pure extract produce 6.5 mm diameter clear zone.
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Affandi, A. A. Fauzia, dan S.D. Lesmana. 2009. Penentuan Dosis Hambat Minimal dan Dosis Bunuh Minimal Larutan Povidon Iodium 10% terhadap Staphylococcus aureus Resisten Metisilin (MRSA) dan Staphylococcus aureus Sensitif Metisilin (MSSA). Jurnal Ilmu Kelautan, 3(1): 14-19
Afriza, D., I. Effendi dan Y.I. Siregar. 2019. Isolation, identification, and Antagonism Test of Heterotrohic Bacteria in Mangrove Plant Against Pathogenic Bacteria (Vibrio alginolyticus, Aeromonas hydrophila, dan Pseudomonas sp. Jurnal Perikanan dan Kelautan, 24(1): 61-68
Avato, P.R., A. Bucci, C. Tava, A. Vitali, Z. Rosato, M. Bialy, & M. jurzysta. 2006. Antimicrobial activity of saponins from medicago spp.: Structure-activity relationship. Phytother. Res. 20: 454-457
Bako, S., I. Lukistyowati, dan H. Syawal. 2019. Sensitivity of Propolis Solutions on Aeromonas Hydrophila bacteria. Jurnal Perikanan dan Kelautan, 24(2): 101-105
Bobbarala, V. 2012. A. Search For Antibacterial Agents. Rizeka. Kroasia. 219 p.
Darlian, L., G. Imran dan Fachrudin. 2011. Skrining Bioaktifitas Kulit Akar Bakau Merah (Rhizophora apiculata) terhadap Daya Hambat Pertumbuhan Koloni Bakteri Streptococcus sp. Jurnal Program Kimia Sains, 1(2): 78-82.
Delmas F., C. Di Giorgio, R. Elias, M. Gasquet, N. Azas, V. Mshvildadadze, G. Dekanosidze, E. Kemertelidze, and P. Timon-David. 2000. Antileishmanial activity of three sapononins isolated from ivy, α-hederin, β-hederin and hederacolchiside Al, as compared to their action on mammalian cells cultured in vitro. Planta Medica, 66:343-347.
Dwijoseputro, D. 2010. Dasar-dasar Mikrobiologi. Jakarta: Djambatan Press.
Frenwick, G.R., K.R. Price, C. Tsukamoto, and K. Okubu. 1991. Saponins. In: Mello, F.J.P.D., Duffus, C.M., Duffus, J.H. (Eds.), Saponins in toxic subtances in Crop Plants. The Royal Seciety of Chemistry, Chambridge.
Hawley, T.S. and R.G. Hawley. 2004. Flow Cytometry Protocols. Human Press, Inc.
Holt. J.G. 2000. Bergey’s Manual Determinatif Bacteriology. Baltimore: Williamn and Wilkins Baltimore.
Huang, H.C. and S.C. Wu. 2003. Moluscicidal saponins from sapindus mukorossi, inhibitory Agents of golden apple snails, Pomacea canalicula. Journal of Agricultural and food chemistry. 51: 4916-4919.
Indobic. 2009. Senyawa Antimikroba dari Tanaman. http//www.indobic.or.id/berita-detail.phpoid_berita=124. Diakses pada tanggal 10 maret 2019.
Kuroda, M., Y. Mimaki, F. Hasegawa, A.Yokosuka, Y. Shasida, & H.
Sakagami. 2001. Steroidal glycosides from the bulbs of Camassia Leichtlinii and their chytotoxic activities. Chemical and Pharmaceutical Bulletin. 49:726-731.
Lukistyowati, I dan Kurniasih. 2012. Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang diberi Pakan Ekstrak Bawang Putih. Jurnal Veteriner, 13(1): 43-50
Lukistyowati, I. 2012. Teknik Pemeriksaan Penyakit Ikan. Pekanbaru: Universitas Riau Press.
Lukistyowati, I. dan Kurniasih. 2011. Kelangsungan Hidup Ikan Mas (Cyprinus carpio) yang diberi Pakan Ekstrak Bawang Putih (Allium sativum) dan Diinfeksi Aeromonas hydrophila. Jurnal Perikanan dan Kelautan. 16(1): 144-160
Mitra, S. and S.R. Dangan. 1997. Miccelar properties of Quillaja saponin.Effects of temperature, salt, and pH on Solution properties. Journal of Agricultural Food Chemistry. 45(5): 1587-1595.
Mulyani.Y, E. Bachtiar, dan M. U. Kurnia. A. 2013. Peranan Senyawa Metabolit Sekunder Tumbuhan Mangrove terhadap Infeksi Bakteri Aeromonas hydrophila pada Ikan Mas (Cyprinus carpio L.). Jurnal Akuatika 4(1): 1-9
Nurjanah, S. B. Prayino dan Sarjito.2014. Sensitivitas Bakteri Aeromonas sp. dan Pseudomonas sp. yang Diisolasi pada Ikan Mas (Cyprinus carpio) Sakit terhadap Berbagai Macam Obat Beredar. Journal of Aquaculture Manajement and Technology. 3(4): 1-9.
Oda, K., H. Matsuda, T. Murakami, S. Katayama, T. Ohgitani, and M. Yoshikawa. 2000. Adjuvant and Haemolytic of 47 Saponins Derived from Medicinal and Food Plants. Biological Chemistry. 381: 67-74.
Peraturan Menteri Kelautan dan Perikanan Repoblik Indonesia. 2012. Obat Ikan. //hhtp://infohukum.kkp.go.id/filespermen/PER%2004%20MEN%202012.pdf. Diakses 5 Mei 2016. 35 hlm.
Sari, N.W., I. Lukistiowaty, dan N. Aryani. 2012. Pengaruh Pemberian Temulawak (Curcuma xanthorriza rxb) terhadap Kelulushidupan Ikan Mas Cyprinus carpio L) Setelah diinfeksi Aeromonas hydrophila. Jurnal Perikanan dan Kelautan, 17(2): 43-59.
Sen, M., H.P.S. Makkar, and K. Becker. 1998. Alfalfa saponins and their implication in animal nutrition. Journal of Agriculture Food Chemistry. 46: 131-140.
Silaban, G.M.P. 2008. Sensitivitas Bakteri Vibrio sp dan Pseudmonas sp. terhadap Ekstrak Buah Pare (Momordica charantia L.). [Skripsi]. Fakultas Perikanan dan Ilmu Kelautan. Universitas Riau. Pekanbaru (Tidak Diterbitkan).
Simatupang, N. dan D. Anggraini. 2013. Potensi Tanaman Herbal sebagai Antimikrobial pada Ikan Lele Sangkuriang (Clarias sp.). Program Studi Budidaya Perairan Fakultas Perikanan Universitas Sriwijaya. Jurnal Akuakultur Rawa Indonesia, 1(2): 216-225
Sumino, A. Supriyadi dan Wardiyanto.2013. Efektivitas Ekstrak Daun Ketapang (Terminalia catappa L.) untuk Pengobatan Infeksi Aeromonas salmonicidapada Ikan Patin (Pangasiodon hypopthalmus). Jurnal Sain Veteriner. 31(1): 79-88
Syawal, H., R. Karnila, A. Dirta, dan R. Kurniawan. 2017. Ekstrak Daun Rhizophora sp Menghambat Pertumbuhan Bakteri Streptococcus agalactiae dan Edwardsiella tarda. Jurnal Veteriner, 18(4): 604-609
Syawal, H., Yuharmen, dan R. Kurniawan. 2019. Sensitivitas Ekstrak Daun Rhizophora apiculata dalam Menghambat Pertumbuhan Bakteri Aeromonas hydrophila. Jurnal Ruaya. 7(2): 34-38
Tampemawa.P.V, J.J. Pelealu, F. E. F. Kandou.2016. Uji Efektifitas Ekstrak Daun Ketapang (Terminalia catappa L.) terhadap Bakteri Bacillus amyloliquefaciens. Jurnal Ilmiah Farmasi. 5(1): 308-320.
Telaumbanua, S., I. Lukistyowati, dan H. Syawal. 2019. Sensitivitas Larutan Biji Mangga Harumanis (Mangifera indica L) terhadap Bakteri Aeromonas hydrophila. Jurnal Perikanan dan Kelautan, 24(1): 24-31
Thanikachalam K., K. Marimutu, and R. Xavier. 2010. Effect of Garlic Peel on Growth, Hematological Parameters and Disease Resistance against Aeromonas hydrophila in African Catfish Clarias gariepinus (Bloch) Fingerling. Asian Pasific Journal of Tropical Medicine. 3: 614-618.
Vincken, J.P., L. Heng, A. De Groot, and J.H. Gruppen. 2007. Saponins, Clasification and occurrence in the plant kingdom. Phytochem. 68:275-297.
Wakabongo, M., B. Enoch B. F.A. Meier and H.P. Dalton. 1992. Rapid Identification of Motil Aeromonas. Diagnostic Microbiology and Infectious Disease 15(6): 511-515.
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DOI: http://dx.doi.org/10.31258/jpk.25.2.116-122
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